2018

From fibroblasts to medicinal signalling cells: a paradigm shift in translational stem cell research
Jonathan Sheard, Thamil Selvee Ramasamy and Darius Widera*
Drug Target Review, 2018 May 31;2018(2): 8-10. 
*corresponding author

Inhibition of NF-κB signalling reduces the stemness characteristics of lung cancer stem cells
Norashikin Zakaria, Narazah Mohd Yusoff, Zubaidah Zakaria, Darius Widera and Badrul H. Yahaya
Front Oncol. 2018 May 17;8:166

Isolation and Characterisation of Neural Crest-Derived Stem Cells from Adult Ovine Palatal Tissue
Marie-Theres Zeuner, Nikolai N. Didenko, David Humphries, Sokratis Stergiadis, Taryn Morash, Ketan Patel, Wolf-Dieter Grimm and Darius Widera*
Front Cell Dev Biol. 2018 Apr 11;6:39. doi: 10.3389/fcell.2018.00039. eCollection 2018. *corresponding author


2017

Quantitative single-molecule imaging of TLR4 reveals ligand-specific receptor dimerization
Carmen L. Krüger, Marie-Theres Zeuner, Graeme S. Cottrell, Darius Widera and Mike Heilemann
Science Signal. 2017 Oct 31;10(503). pii: eaan1308. doi: 10.1126/scisignal.aan1308.

Toll-like Receptor 4 Signalling and its Impact on Platelet Function, Thrombosis, and Haemostasis
Thomas Vallance, Marie-Theres Zeuner, Harry F. Williams, Darius Widera* and Sakthivel Vaiyapuri*
Mediators Inflamm. 2017;2017:9605894. doi 10.1155/2017/9605894.  *corresponding authors

3D Cell Culture of Human Mesenchymal Stem Cells in Nanofibrillar Cellulose Hydrogels
Ioannis Azoidis, Joel Metcalfe, James Reynolds, Shirley Keeton, Sema Hakki, Jonathan Sheard and Darius Widera*
MRS Communications. 2017 Aug 11, doi:10.1557/mrc.2017.59. [Epub ahead of print]*corresponding author

Protein and molecular characterisation of a clinically compliant amniotic fluid stem cell derived extracellular vesicle fraction capable of accelerating muscle regeneration through the enhancement of angiogenesis
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chambers, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Steve Ray, Darius Widera, Anna L David, Tobias B. Huber, Joern Dengjel, Paolo De Coppi and Ketan Patel
Stem Cells Dev. 2017 Jul 5. doi: 10.1089/scd.2017.0089. [Epub ahead of print]

Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation
Marie-Theres Zeuner, Thomas Vallance, Sakthivel Vaiyapuri, Graeme S. Cottrell and Darius Widera*
Mediators Inflamm. 2017;2017:6209865. doi: 10.1155/2017/6209865. Epub 2017 Jul 16.  *corresponding author

The Use of Mesenchymal Stromal Cells in Treatment of Lung Disorders
Egi Kardia, Norashikin Zakaria, Nur Shuhaidatul Sarmiza Abdul Halim, Darius Widera and Badrul Hisham Yahaya
Regen Med. 2017 Mar;12(2):203-216. doi: 10.2217/rme-2016-0112.


2016

Biased Signalling is an Essential Feature of TLR4 in Glioma Cells
Marie-Theres Zeuner, Carmen L Krüger, Katharina Volk, Karen Bieback, Graeme S Cottrell, Mike Heilemann and Darius Widera*
Biochim Biophys Acta. 2016 Sep 23;1863(12):3084-3095. doi: 10.1016/j.bbamcr.2016.09.016, *corresponding author

Single-Particle Tracking Uncovers Dynamics of Glutamate-Induced Retrograde transport of NF-κB p65 in living neurons
Darius Widera*, Christin Klenke, Deepak Nair, Meike Heidbreder, Sebastian Malkusch, Jean-Baptiste Sibarita, Daniel Choquet, Barbara Kaltschmidt, Mike Heilemann*, Christian Kaltschmidt*
Neurophotonics. 2016 Oct;3(4):041804. doi: 10.1117/1.NPh.3.4.041804 *corresponding authors

1,8-cineole potentiates IRF3-mediated antiviral response in human stem cells and an ex vivo model of rhinosinusitis
Janine Müller, Johannes FW Greiner, Marie-Theres Zeuner, Viktoria Brotzmann, Johanna Schafermann, Frederique Wieters, Darius Widera, Holger Sudhoff, Barbara Kaltschmidt and Christian Kaltschmidt
Clin Sci (Lond). 2016 Aug 1;130(15):1339-52. doi: 10.1042/CS20160218.

Paracrine effects of TLR4-polarised mesenchymal stromal cells are mediated by extracellular vesicles
Marie-Theres Zeuner, Ketan Patel, Bernd Denecke, Bernd Giebel and Darius Widera*
J Transl Med. 2016 Feb 2;14(1):34. doi: 10.1186/s12967-016-0794-z. *corresponding author


2015

Helium Ion Microscopy Visualizes Lipid Nanodomains in Mammalian Cells
Matthias Schürmann, Natalie Frese, André Beyer, Peter Heimann, Darius Widera, Viola Mönkemöller, Thomas Huser, Barbara Kaltschmidt, Christian Kaltschmidt, Armin Gölzhäuser
Small. 2015 Oct 5. doi: 10.1002/smll.201501540

Translational Research and Therapeutic Applications of Neural Crest-Derived Stem Cells in Regenerative Periodontology
Wolf-Dieter Grimm, Bernd Giesenhagen, Sema Hakki, Ingmar Schau, Sergey Sirak, Alexandr Sletov, Gabor Varga, Alexander Vukovic and Darius Widera
Current Oral Health Reports, Stem Cell Biology For Tooth And Periodontal Regeneration, 2015 Sep 23. doi: 10.1007/s40496-015-0067-6

Controversial Role of Toll-like Receptor 4 in Adult Stem Cells
Marie Zeuner, Karen Bieback and Darius Widera
Stem Cell Rev. 2015 Aug;11(4):621-34. doi: 10.1007/s12015-015-9589-5.

Alternative generation of CNS neural stem cells and PNS derivatives from neural crest derived peripheral stem cells
Marlen Weber, Galina Apostolova, Darius Widera, Michel Mittelbronn, Georg Dechant, Barbara Kaltschmidt and Hermann Rohrer
Stem Cells. 2015 Feb;33(2):574-88. doi: 10.1002/stem.1880.

Intrastriatal transplantation of adult human neural crest-derived stem cells improves functional outcome in Parkinsonian rats
Janine Müller, Christiana Ossig, Johannes F.W. Greiner, Stefan Hauser, Mareike Fauser, Darius Widera, Christian Kaltschmidt, Alexander Storch and Barbara Kaltschmidt
Stem Cells Transl Med. 2015 Jan;4(1):31-43. doi: 10.5966/sctm.2014-0078.


2014

Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation
Matthias Schürmann, Annalena Wolff, Darius Widera, Stefan Hauser, Peter Heimann, Andreas Hütten, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cell Res. 2014 May 9;13(1):98-110. doi: 10.1016/j.scr.2014.04.017. [Epub ahead of print]

Culture bag systems for clinical applications of adult human neural crest-derived stem cells
Lena-Marie Grunwald, Johannes FW Greiner, Janine Müller, Darius Widera, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cell Res Ther. 2014 Mar 14;5(2):34. [Epub ahead of print]

Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization towards higher order oligomers
Franziska Fricke, Sebastian Malkusch, Gaby Wangorsch, Johannes F. Greiner, Barbara Kaltschmidt, Christian Kaltschmidt , Darius Widera, Thomas Dandekar , Mike Heilemann
Histochem Cell Biol. 2014 Feb 12. [Epub ahead of print]

Methods for Modulation and Analysis of NF-kappaB-dependent Adult Neurogenesis
Darius Widera, Yvonne Imielski, Christian Kaltschmidt and Barbara Kaltschmidt
J Vis Exp. 2014 Feb 13;(84). doi: 10.3791/50870.

An Investigation of the Specificity of Research Antibodies against NF-κB-subunit p65
Carsten Slotta, Janine Müller, Loan Tran, Stefan Hauser, Darius Widera, Barbara Kaltschmidt and Christian Kaltschmidt
J Histochem Cytochem. 2014 Feb;62(2):157-61


2013

Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells
The Duy Nguyen*, Darius Widera*, Johannes Greiner, Janine Müller, Ina Martin, Carsten Slotta, Stefan Hauser, Christian Kaltschmidt and Barbara Kaltschmidt
Biol Chem. 2013 Dec;394(12):1623-36. doi: 10.1515/hsz-2013-0191. *equal contribution

1,8-Cineol inhibits nuclear translocation of NF-κB p65 and NF-κB-dependent transcriptional activity.
Johannes F.-W. Greiner, Janine Müller, Marie-Theres Zeuner, Thorsten Seidel, Christin Zander, Stefan Hauser, Darius Widera, Holger Sudhoff, Barbara Kaltschmidt and Christian Kaltschmidt
Biochim Biophys Acta. 2013 Dec;1833(12):2866-78.

Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.
Anje Sporbert, Meike Heidbreder, Petra Domaing, Stefan Hauser, Zoltan Cseresnyes, Barbara Kaltschmidt, Christian Kaltschmidt, Mike Heilemann and Darius Widera*

PLoS ONE 2013 Mai 21; 8(5): e64023. doi:10.1371/journal.pone.0064023. *corresponding author

Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons
Christin Klenke, Darius Widera *, Thomas Engelen, Janine Müller, Thomas Noll, Karsten Niehaus, M. Lienhard Schmitz, Barbara Kaltschmidt and Christian Kaltschmidt
PLoS One. 2013 Jun 7;8(6):e65280. doi: 10.1371/journal.pone.0065280. Print 2013. *equal contribution

Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell xenografts
Timo Schomann, Firas Qunneis, Darius Widera, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cells Int, 2013,2013, http://dx.doi.org/10.1155/2013/960958

An intronic G run within HIV-1 intron 2 is critical for splicing regulation of vif mRNA
Marek Widera, Steffen Erkelenz, Frank Hillebrand, Aikaterini Krikoni, Darius Widera, Wolfgang Kaisers, René Deenen, Michael Gombert, Rafael Dellen, Tanya Pfeiffer, Barbara Kaltschmidt, Carsten Münk, Valerie Bosch, Karl Köhrer, and Heiner Schaal
J Virol. 2013 Mar;87(5):2707-20. doi: 10.1128/JVI.02755-12. Epub 2012 Dec 19.


2012

Adult Craniofacial Stem Cells: Sources and Relation to the Neural Crest
Barbara Kaltschmidt, Christian Kaltschmidt and Darius Widera *
Stem Cell Rev. 2012 Sep;8(3):658-71. *corresponding author

Book Chapter: TNF receptor membrane dynamics studied with fluorescence methods.
Felix Neugart, Darius Widera*, Barbara Kaltschmidt, Christian Kaltschmidt and Mike Heilemann*.
Fluorescent Methods to Study Biological Membranes.-Springer Series on Fluorescence, DOI:10.1007/4243_2012_60 2012, 439-457 *corresponding authors

Identification of Novel Cholesteatoma-related Gene Expression Signatures Using Full-genome Microarrays
Christin Zander, Sebastian Janowski, Daniela Borck, Darius Widera, Jörg Ebmeyer, Jörn Kalinowski, Anke Leichtle, Ralf Hofestädt, Tahwinder Upile, Christian Kaltschmidt, Barbara Kaltschmidt and Holger Sudhoff
PLoS One
. 2012;7(12):e52718. doi: 10.1371/journal.pone.0052718. Epub 2012 Dec 20.

Label-free enrichment of leucozytozoids using flow cytometric cell sorting
Johannes Greiner, Nayden Chakarov, Stefan Hauser, Daniel Schützmann, Martina Boerner, Oliver Krüger and Darius Widera*
Parasitology. 2012 Oct;139(12):1547-52. doi: 10.1017/S0031182012000972. Epub 2012 Jul 19. *corresponding author

Generation of Schwann cell-derived multipotent neurospheres isolated from intact sciatic nerve
Ina Martin, The Duy Nguyen, Vivien Krell, Johannes F.W. Greiner, Janine Müller, Peter Heimann and Darius Widera*
Stem Cell Rev. 2012 Dec;8(4):1178-87. doi: 10.1007/s12015-012-9387-2. *corresponding author

Orgin and regenerative potential of vertebrate mechanoreceptor-associated stem cells
Darius Widera, Stefan Hauser, Christian Kaltschmidt and Barbara Kaltschmidt
Anatomy Research International, vol. 2012, 2012, doi:10.1155/2012/837626

TNF-a alpha influences the lateral dynamics of TNF receptor I in living cells
Meike Heidbreder, Christin Zander, Sebastian Malkusch Darius Widera, Barbara Kaltschmidt, Christian Kaltschmidt, Deepak Nair, Jean-Baptiste Sibarita, Daniel Choquet, Mike Heilemann
Biochim Biophys Acta Molecular Cell Research. 2012 Oct;1823(10):1984-9. Epub 2012 Jun 27.

Isolation of Novel Multipotent Neural Crest-Derived Stem Cells from Adult Human Inferior Turbinate
Stefan Hauser, Darius Widera, Firas Qunneis, Janine Müller, Christin Zander, Johannes Greiner, Christina Strauss, Patrick Lüningschrör, Peter Heimann, Holger Sudhoff, Marcos J. Araúzo-Bravo, Boris Greber, Holm Zaheres, Hans Schöler, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cells Dev. 2012 Mar 20;21(5):742-56. Epub 2012 Jan 26.


2011

Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics
Johannes Greiner, Stefan Hauser, Darius Widera, Firas Qunneis, Janine Müller, Christin Zander, Ina Martin, Jana Mallah, Christian Prante, Hartmut Schwarze, Wolfgang Prohaska, André Beyer, Karsten Rott, Andreas Hütten, Armin Gölzhäuser, Holger Sudhoff, Christian Kaltschmidt and Barbara Kaltschmidt
Eur Cell Mater. 2011 Dec 17;22:403-19.

Schwann Cells Can Be Reprogrammed to Multipotency by Culture
Darius Widera, Peter Heimann, Christin Zander, Yvonne Imielski, Meike Heidbreder, Mike Heilemann, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cells Dev. 2011 Dec;20(12):2053-64. Epub 2011 Jun 1.


2010

Subdiffraction Fluorescence Imaging of Biomolecular Structure and Distributions with Quantum Dot Triexciton Imaging (QDTI)
Meike Heidbreder, Ulrike Endesfelder, Sebastian van de Linde, Simon Hennig, Darius Widera, Barbara Kaltschmidt, Christian Kaltschmidt and Mike Heilemann
Biochim Biophys Acta Molecular Cell Research. 2010 Oct;1803(10):1224-9. Epub 2010 Jun 23.

Morphological characterization of periodontium-derived human stem cells
Wolfgang Arnold, Sebastian Becher, Aous Dannan, Darius Widera, Thomas Dittmar, Monica Jacob, Hans Georg Mannherz, Barbara Kaltschmidt, Christian Kaltschmidt, Wolf-Dieter Grimm
Ann Anat. 2010 Aug 20;192(4):215-9. Epub 2010 Jun 11.

Neural stem cells adopt tumorigenic properties by constitutively activated NF-kappaB and subsequent VEGF up-regulation
Aljoscha Kaus*, Darius Widera*, Susannah Kassmer, Jan Peter, Kurt Zaenker, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cells Dev. 2010 Jul;19(7):999-1015,*equal contribution


2009

Adult palatum as a novel source of neural crest-related stem cells
Darius Widera *, Christin Zander*, Meike Heidbreder, Yvonne Kasperek, Thomas Noll, Oliver Seitz, Belma Saldamli, Holger Sudhoff H, Robert Sader, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cells.
2009 Aug;27(8):1899-910. * equal contribution

Book Chapter: NF-kappaB, Potential Role in Adult Neural Stem Cells
Darius Widera, Christian Kaltschmidt and Barbara Kaltschmidt
In: Binder, Hirokawa, Windhorst, Hirsch (Eds.) Encyclopedia of Neuroscience. Springer Verlag, Berlin, 2009: 2862 - 2867.


2008

Neural stem cells, inflammation and NF-kappaB: basic principle of maintenance and repair or origin of brain tumours?
Darius Widera, Aljoscha Kaus, Ilja Mikenberg, Christian Kaltschmidt and Barbara Kaltschmidt
J Cell Mol Med. 2008 Mar-Apr;12(2):459-70. Epub 2007 Dec 20.


2007

Impact of polysialylated CD56 on natural killer cell cytotoxicity
Jeannette M. Moebius, Darius Widera, Jürgen Schmitz, Christian Kaltschmidt and Christoph Piechaczek
BMC Immunology, 2007 Aug 6;8(1):13

Transcription factor NF-kappaB is transported to the nucleus via cytoplasmic dynein/dynactin motor complex in hippocampal neurons
Ilja Mikenberg, Darius Widera, Aljoscha Kaus, Barbara Kaltschmidt and Christian Kaltschmidt
PLOS One, 2007 Jul 11;2:e589

Highly Efficient neural differentiation of human somatic stem cells, isolated via minimally-invasive periodontal surgery
Darius Widera, Wolf Dieter Grimm, Jeannette M. Moebius, Ilja Mikenberg, Christoph Piechaczek, Georg Gassmann, Natascha A Wolff, Frank Thévenod, Christian Kaltschmidt and Barbara Kaltschmidt
Stem Cells Dev. 2007 Jun;16(3):447-60.


2006

Mathematical model for NF-kappaB driven proliferation of adult neural stem cells
Monika Piotrowska*, Darius Widera *, Barbara Kaltschmidt, Uwe an der Heiden and Christian Kaltschmidt
Cell Prolif, 39(6):441-455. Epub 2006 Nov 15, *equal contribution

Tumor Necrosis Factor alpha triggers proliferation of adult neural stem cells via IKK/NF-kappaB signaling
Darius Widera, Ilja Mikenberg, Margitta Elvers, Christian Kaltschmidt and Barbara Kaltschmidt
BMC Neuroscience 2006 Sep 20; 7:64

Potential Role of NF-kappaB in Adult Neural Stem Cells: the Underrated Steersman?
Darius Widera, Ilja Mikenberg, Barbara Kaltschmidt and Christian Kaltschmidt
Int J Dev Neurosci. 2006 Apr-May;24(2-3):91-102. Epub 2006 Jan 18

Long-term depression activates transcription of immediate early transcription factor genes: involvement of SRF/Elk-1
Antje Lindecke, Martin Korte, Marta Zagrebelsky, Volker Horejschi, Margitta Elvers,
Darius Widera, Maria Prüllage, Julia Pfeiffer, Barbara Kaltschmidt and Christian Kaltschmidt
Eur J Neuroscience, 2006 Jul;24(2):555-63

Nuclear Factor-kappaB controls the reaggregation of 3D neurosphere cultures in vitro
Darius Widera, Ilja Mikenberg, Aljoscha Kaus, Christian Kaltschmidt and Barbara Kaltschmidt
Eur Cell Mater. 2006 May 23;11:76-85

TNF-alpha mediated transport of NF-kappaB to the nucleus is independent of the cytoskeleton-based transport system in non-neuronal cells
Ilja Mikenberg,
Darius Widera, Aljoscha Kaus, Barbara Kaltschmidt and Christian Kaltschmidt
Eur J Cell Biol. 2006 Jun;85(6):529-36. Epub 2006 Apr 3


2005

Signalling via NF-kB in the nervous system
Barbara Kaltschmidt, Darius Widera and Christian Kaltschmidt
Biochim Biophys Acta. 2005 Sep 30;1745(3):287-99

Book Chapter: The role of NF-kappaB in brain function
Barbara Kaltschmidt, Ilja Mikenberg, Darius Widera and Christian Kaltschmidt
In: Thiel (Ed.) Transcription Factors in the Nervous System, Wiley-VCH, Weinheim, 2005


2004

MCP-1 induces migration of adult neural stem cells
Darius Widera, Wolf Holtkamp, Frank Entschladen, Bernd Niggemann, Kurt Zänker, Barbara Kaltschmidt, and Christian Kaltschmidt
Eur J Cell Biol. 2004 Aug;83(8):381-7